POTENTIAL HERB-DRUG INTERACTION OF DECALEPIS HAMILTONII VIA P-GP MEDIATED PHARMACOKINETIC INTERACTION WITH FEXOFENADINE IN RATS: AN IN SITU AND IN VIVO STUDY

Objective: The objective of this study was to investigate the influence Decalepis hamiltonii (D. hamiltonii), a traditional plant used in herbal medicine, on intestinal absorption and pharmacokinetics fexofenadine, substrate P-glycoprotein (P-gp), rats. Methods: In situ perfusion tests were conducted assess permeability fexofenadine. P-gp ATPase activity also evaluated understand modulatory effects D. P-gp. An vivo pharmacokinetic investigation performed by administering oral fexofenadine Results: results revealed that effective permeation (Peff) significantly diminished (p<0.001***) aqueous extract (AREDH, 200 mg/kg p. o.) pretreated group compared normal control indicating modulation absorption. Further, there significant augmentation (p<0.01**) AREDH (200 inductive potential hamiltonii. Pharmacokinetic peak plasma concentration (Cmax) area under concentration-time curve (AUC) downregulated compromised bioavailability. However, no changes observed half-life (T1/2 k10), time reach (Tmax), or elimination rate constant (k10). Conclusion: conclusion, reduced bioavailability promoting P-gp-mediated drug efflux during This suggests characteristics may lead herb-drug interactions when taken combination with xenobiotics, emphasizing importance considering such clinical practice further research.